Session: All You Need to Know About Gene Silencing: RNAi in vitro and in vivo


Room 204C (Washington DC Convention Center)
All You Need to Know About Gene Silencing: RNAi in vitro and in vivo
We will present experimental data that clearly demonstrates that effective silencing of gene relevant to pain by means of post-transcriptional degradation of homologous mRNA changes inflammatory response, cell function and nociceptive behaviors. Dr. JASMIN will describe the technology of gene silencing using RNA inhibition (RNAi) and its potential applications in patients. Dr. BHARGAVA will review the basic principles of RNAi and show how to get it to work in vivo. She will show clear evidence of quantifiable gene suppression of the coricotropin-releasing factor (CRF) gene both in the hypothalamus and in the intestine. She will show how inhibition of the CRF gene blocks the inflammatory response. Dr. YANG will show how short hairpin RNA can be made and incorporated in lentivirus to ensure long-term gene suppression. He will show that suppression of the NaV1.8 tetrodotoxin-resistant voltage gated channel Na current density without changes in other biophysical properties as measure with patch clamp recordings. Dr. OHARA will show a practical application of RNAi that could lead to the use of this technology in clinical practice. He will show that silencing of a glial specific potassium channel, the Kir4.1 channel, lead to increased pain behavior in the rat trigeminal system. The results show a close correlation between the change in behavior and the change in expression of the Kir4.1 channel while the expression of other genes is not altered. These results show that that changes in pain behavior can be brought about by changes to a single ion channel in glia cells in sensory ganglia.

Moderator:	Luc Jasmin, MD, PhD
Presenters:	Aditi Bhargava, PhD Jay Yang, MD, PhD Peter T. Ohara, PhD
		All You Need to Know About Gene Silencing: RNAi in vitro and in vivo Luc Jasmin, MD, PhD
		All You Need to Know About Gene Silencing: RNAi in vitro and in vivo-1 [Handout] [Recording] Aditi Bhargava, PhD
		The nuts & bolts for incorporating siRNA into your pain research [Handout] Jay Yang, MD, PhD
		In vivo application of RNAito target single genes in the trigeminal ganglion related to pain [Handout] [Recording] Peter T. Ohara, PhD

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